热辣2号辣椒纯度鉴定及优良自交系遗传多样性分析

为了提高种子质量和充分利用辣椒种质资源,应用SSR分子标记对热辣2号杂交种纯度进行鉴定并对部分辣椒优良自交系进行遗传多样性分析。以热辣2号母本材料CAYB02和父本材料CAYB01为模板筛选85对辣椒SSR引物,其中12对引物在亲本间能扩增出明显的多态性,多态性比率为14.12%,多态性标记分别位于辣椒1、3、4、6、7、9、11、12号染色体,利用3对位于不同染色体上的SSR标记对热辣2号黄灯笼椒杂交种的纯度进行鉴定,热辣2号种子纯度为98.79%,标记鉴定结果与田间鉴定结果一致。研究结果表明,利用SSR标记鉴定辣椒杂交种纯度是切实可行的。利用12对多态性明显、稳定可靠的SSR引物对部分辣椒自交系进行遗传多样性分析,共扩增出60条条带,多态性位点比率为100%,平均每对引物检测出5个等位基因。30份辣椒自交系间遗传相似系数变幅为0.33~1.00,平均为0.65,表明供试材料间具有丰富的遗传多样性。聚类分析结果表明,在遗传相似系数为0.47时,可以将中国辣椒和一年生辣椒区分开来。在遗传相似系数为0.85时,30份辣椒优良自交系分为11个类群,辣椒种质遗传关系与地理来源和农艺性状具有一定的相关性。     

Effect of herbicide application on weed flora under conservation agriculture in Zimbabwe

Increased challenges of weed control in the smallholder farming sector of southern Africa have often resulted in small yields. The objective of this study was to evaluate the effects of different weed control strategies on weed flora and composition under conservation agriculture (CA) systems in Zimbabwe. This study was conducted at three on-station trial sites namely Domboshawa Training Centre (DTC), University of Zimbabwe farm (UZ farm) and Henderson Research Station (HRS) in a maize–soybean rotation for four seasons from 2009–2010 to 2012–2013 seasons. Hand weeding was done whenever weeds were 10 cm tall or 10 cm in circumference for weeds with a stoloniferous growth habit. Weed identification was done up to the weed species level, and the Shannon–Weiner diversity and evenness index was used to determine the response of weed flora to herbicides. Results showed that there were more weeds in the early years which decreased gradually until the final season. Weed species diversity was not affected by herbicide application and the results indicated that weed species diversity was small in CA systems. Annual weed species constituted a greater proportion of species, and species richness decreased with the duration of the study. Richardia scabra L. and Galinsoga parviflora Cav. were the most common dominant weed species at all sites and in all seasons. Moreover, herbicide application had no effect on the evenness of weeds in the plots but site characteristics had a significant effect on the distribution of weed species (weed species evenness). The results presented in this study suggest that herbicide application facilitates a depletion of weed seed bank/number of weeds over time. Thus, herbicide application in CA has potential to reduce weed density, species richness and species diversity in the long term which may lead to more labour savings and larger yields.     

5个冬麦品种产量性状和叶片特征多样性分析

采用单因素完全随机设计,调查5个冬麦品种产量性状和叶片特征多样性。结果表明,5项产量相关性状指标(株高、穗长、主穗粒数、有效分蘖数、有效小穗数)在不同冬麦品种间差异均达显著水平;5个品种的上表皮气孔密度均多于下表皮,其中,上表皮气孔密度为62.67~71.11个/mm2,下表皮气孔密度为30.00~46.25个/mm2;叶脉总数为57.00~65.67条;叶脉密度为36.89~43.00条/mm。     

刈割强度对大针茅草原植物群落特征和土壤理化性质的影响

探究不同刈割强度对大针茅典型草原植物群落特征以及土壤理化性质的影响,可为大针茅草原制定合理科学的刈割制度提供理论依据。本研究以大针茅典型草原为对象,于2020年在内蒙古锡林浩特市毛登牧场刈割实验平台进行野外植被调查和取样。该实验平台于2014年建立,以不刈割为对照（CK）,设置轻度（LM）、中度（MM）及重度刈割（HM）3个处理。本研究通过计算物种重要值和多样性指数,测定植物群落地上、地下生物量以及土壤理化性质,探究群落特征与土壤理化性质的关系。结果表明：1）与不刈割相比,不同刈割强度均增加了新物种,且中度刈割的物种数最多;与不刈割相比,不同刈割强度均显著增加了群落的丰富度和Shannon-Wiener指数,且轻度刈割显著增加了优势度和均匀度指数,表明轻度刈割最有利于群落多样性的维持;不同刈割强度对大针茅草原的生产力均无显著影响;2）土壤全碳、全氮和pH均在中度刈割时最高,且显著高于不刈割,表明中度刈割对土壤养分有积极影响;3）硝态氮是影响草地生产力的主要土壤因子,土壤含水量是影响草地群落多样性的主要土壤因子。本研究可为大针茅典型草原群落结构、物种多样性以及生产力等方面的研究提供重要参...     

短期围栏封育对高寒草甸植物群落及土壤理化性质的影响

充分了解围栏封育对植物群落及土壤理化性质的影响,是合理制定草甸恢复和利用相关政策、促进当地畜牧业经济可持续发展的关键。本研究通过对西藏三江并流区封育2年的高寒草甸的群落样方调查与土壤理化性质测定,结果表明,比较自由放牧,围封后地上和地下生物量分别提高26.24%和8.94%,但物种多样性指数略有下降;围封后土壤电导率、盐度和总溶解固体分别下降26.50%,29.03%,30.15%;围栏内地上生物量与土壤盐度、总溶解固体、电导率呈显著正相关(P<0.05),地下生物量与硝态氮、温度呈显著正相关(P<0.05);围栏外地下生物量与介电常数、体积含水率呈显著负相关(P<0.05),与铵态氮呈显著正相关(P<0.05)。研究结果表明,2年短期围封对高寒草甸植物群落及其土壤理化性质均有一定改善,可以作为应对草甸退化的干扰手段与管理措施。     

放牧和模拟降水对短花针茅荒漠草原植物功能群多样性的影响

为探究放牧和降水变化对植物功能群的影响,本文以内蒙古短花针茅(Stipa breviflora)荒漠草原为研究对象,开展了野外控制试验。试验设置围封和适度放牧,分别进行模拟降水处理(减少降水量50%、自然降水、增加降水量50%、增加降水量100%),并在每年8月观测植物群落组成。结果表明：减水会降低植物功能群多样性、生物量和物种数量,增水有利于植物功能群多样性的提高。多年生丛生禾草多样性随降水量的增加而升高。同时,对降水梯度和功能群多样性进行拟合分析发现,降水量低于年降水量16%则不利于除灌木、半灌木外其他功能群植物的生长。围封草地增水67%～79%时,功能群的Simpson指数、Shannon-Wiener指数、Pielou指数值均较高,功能群多样性较高;由于放牧提高了植物的水分利用效率,放牧地各指数在增水26%～50%时较高,功能群多样性较高。综上,适度的放牧及水分补充有利于提高植物功能群多样性。     

生物多样性与地形及土壤因素对坡面产流产沙的耦合影响

生物多样性关系到生态系统的功能,而坡度和土壤因素是影响坡面产流产沙的重要因子。本研究基于黄土丘陵区生物多样性,并引入坡度、降雨前土壤含水量和土壤容重,探究其与坡面产流产沙总量的耦合关系。通过野外人工降雨试验,记录草本、灌木和乔木群落下的产流时间和产流产沙总量,并开展土壤植被调查。结果表明,与草本和乔木群落相比,灌木群落在延长产流开始时间和降低产沙总量上表现更优;植被群落功能离散度指数(FDiv)对产沙总量影响最大,Simpson指数(D)对产流总量影响最大,坡度仍然是不可忽视的第二重要影响因素;产流总量与坡度、土壤容重、D和功能丰富度指数(FRic)的回归关系优于产流总量分别与其的回归关系;产沙总量与坡度和FDiv指数的回归关系优于产沙总量分别与其的回归关系。植被保护与坡度改善将是该区水土保持规划工作的重点。     

Using genetic tools to inform conservation of fragmented populations of Asian elephants (Elephas maximus) across their range in China

A herd of 15 Chinese elephants attracted international attention during their 2021 northward trek, motivating the government to propose establishment of an Asian elephant national park. However, planning is hampered by a lack of genetic information on the remaining populations in China. We collected DNA from 497 dung samples from all 5 populations encompassing the entire range of elephants in China and used mitochondrial and microsatellite markers to investigate their genetic and demographic structure. We identified 237 unique genotypes (153 females, 84 males), representing 81% of the known population. However, the effective population size was small (28, range 25–32). Historic demographic contraction appeared to account for low haplotype diversity (H_d = 0.235), but moderate nucleotide and nuclear diversity (π = 0.6%, H_e = 0.55) was attributable to post-bottleneck recovery involving recent population expansion plus historical gene exchange with elephants in Myanmar, Lao PDR, and Vietnam. The 5 populations fell into 3 clusters, with Nangunhe elephants differing consistently from the other 4 populations (F_ST = 0.23); elephants from Mengyang, Simao, and Jiangcheng belonged to a single population (henceforth, MSJ), and differed from the Shangyong population (F_ST = 0.11). Interpopulation genetic variation reflected isolation by distance and female-biased dispersal. Chinese elephants should be managed as 2 distinct units: Nangunhe and another combining Shangyong and MSJ; their long-term viability will require restoring gene flow between Shangyong and MSJ, and between elephants in China and neighboring countries. Our results have the potential to inform conservation planning for an iconic megafaunal species.     

哺乳期幼驼与成年母驼粪便菌群多样性分析

【目的】探究准噶尔双峰驼哺乳期幼驼和成年母驼粪便菌群结构组成及多样性变化,为幼驼肠道健康发育提供重要依据。【方法】选取6~7岁体况相近的健康成年母驼12峰,以及出生日期相近（3月龄）的哺乳期母幼驼12峰,母驼与幼驼在相同饲养环境下饲养。采用直肠取粪法采集粪样,用于粪便内容物细菌16S rDNA的V3-V4区测序,并对测序数据进行相应分析。【结果】母驼粪便菌群Chao1和Shannon指数均显著高于幼驼（P<0.05）;在门水平上,母驼和幼驼粪便中位于前十的菌均为厚壁菌门、拟杆菌门、疣微菌门、变形菌门、软壁菌门、螺旋体门、TM7、放线菌门、蓝藻菌门和纤维杆菌门,其中幼驼粪便中变形菌门和软壁菌门丰度均极显著高于母驼（P<0.01）;在科水平上,母驼与幼驼粪便中位于前十的菌均为瘤胃球菌科、消化链球菌科、毛螺菌科、梭菌科、克里斯滕森菌科、理研菌科、疣微菌科、拟杆菌科、艰难杆菌科和普雷沃氏菌科,其中母驼粪便中消化链球菌科和梭菌科的丰度均极显著高于幼驼（P<0.01）,而幼驼粪便中毛螺菌科的丰度极显著高于母驼（P<0.01）;在属水平上,母驼与幼驼粪便中位于前十的菌属均为梭菌属、颤螺旋菌属、瘤胃球菌属、梭菌科-梭菌属、阿克曼菌属、5-7 N15、CF231、苏黎世杆菌属、梭菌科-SMB53和密螺旋体菌属,其中母驼粪便中的梭菌属、梭菌科-梭菌属和苏黎世杆菌属的丰度均极显著高于幼驼（P<0.01）。【结论】成年母驼粪便菌群多样性显著高于哺乳期幼驼,且稳定性较好;母驼和幼驼的粪便在门、科、属水平下的主导菌属种类均一致,但是丰度均存在差异。